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Y-family DNA polymerases in Escherichia coli

By Daniel F. Jarosz, Penny J. Beuning, Susan E. Cohen and Graham C. Walker

Abstract

The observation that mutations in the Escherichia coli genes umuC+ and umuD+ abolish mutagenesis induced by UV light strongly supported the counterintuitive notion that such mutagenesis is an active rather than passive process. Genetic and biochemical studies have revealed that umuC+ and its homolog dinB+ encode novel DNA polymerases with the ability to catalyze synthesis past DNA lesions that otherwise stall replication – a process termed translesion synthesis (TLS). Similar polymerases have been identified in nearly all organisms, constituting a new enzyme superfamily. Although typically viewed as unfaithful copiers of DNA, recent studies suggest that certain TLS polymerases can perform proficient and moderately accurate bypass of particular types of DNA damage. Moreover, various cellular factors can modulate their activity and mutagenic potential.National Institutes of Health (U.S.) (grant CA021615)National Institute of Environmental Health Sciences (Grant P30 ES002109

Publisher: 'Elsevier BV'
Year: 2007
DOI identifier: 10.1016/j.tim.2006.12.004
OAI identifier: oai:dspace.mit.edu:1721.1/70041
Provided by: DSpace@MIT
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