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Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation

By F. P. Sweeney, Orin Courtenay, V. Hibberd, R. G. Hewinson, L. A. Reilly, William H. Gaze and E. M. H. Wellington


Real-time PCR was used to detect and quantify Mycobacterium bovis cells in\ud naturally infected soil and badger faeces. Immunomagnetic capture,\ud immunofluorescence and selective culture confirmed species identification and cell\ud viability. These techniques will prove useful for monitoring M. bovis in the\ud environment and for elucidating transmission routes between wildlife and cattle

Topics: QR
Publisher: American Society for Microbiology
Year: 2007
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