Location of Repository

EPR spectroscopy and its use in planta - a promising technique to disentangle the origin of specific ROS

By Anja eSteffen-Heins and Bianka eSteffens

Abstract

While it is widely accepted that reactive oxygen species (ROS) are common players in developmental processes and a large number of adaptations to abiotic and biotic stresses in plants, we still do not know a lot about ROS level control at cellular or organelle level. One major problem that makes ROS hard to quantify and even to identify is their short lifetime. A promising technique that helps to understand ROS level control in planta is the electron paramagnetic resonance (EPR) spectroscopy. Application of the spin trapping method and the spin probe technique by this advanced method enables the quantification and identification of specific ROS in different plant tissues, cells or organelles or under different conditions. This mini review summarizes the knowledge using EPR spectroscopy as a method for ROS detection in plants under different stress conditions or during development. This technique allows disentangling the origin of specific ROS and transient alteration in ROS levels that occur by changes in ROS production and scavenging

Topics: reactive oxygen species (ROS), ROS detection, Spin trap, Spin probe, electron paramagnetic resonance spectroscopy (EPR), Environmental sciences, GE1-350
Publisher: Frontiers Media S.A.
Year: 2015
DOI identifier: 10.3389/fenvs.2015.00015
OAI identifier: oai:doaj.org/article:91693029250c4b4aa326c6d36374818d
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/2296-665X (external link)
  • http://journal.frontiersin.org... (external link)
  • https://doaj.org/article/91693... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.