Location of Repository

Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species

By Ana M. Ferreira, Ana M. Ferreira, Andreia T. Marques, Luca eFontanesi, Carl-Gustaf eThulin, Elvira eSales-Baptista, Susana S. Araújo, Susana S. Araújo and André M. Almeida

Abstract

The repertoires of bitter taste receptor (T2R) gene have been described for several animal species, but these data are still scarce for Lagomorphs. The aim of the present work is to identify potential repertoires of T2R in several Lagomorph species, covering a wide geographical distribution. We studied these genes in Lepus timidus, Lepus europaeus, Oryctolagus cuniculus algirus, Romerolagus diazi and Sylvilagus floridanus, using Oryctolagus cuniculus cuniculus as control species for PCR and DNA sequencing. We studied the identities of the DNA sequences and built the corresponding phylogenetic tree. Sequencing was successful for both subspecies of Oryctolagus cuniculus for all T2R genes studied, for five genes in Lepus, and for three genes in Romerolagus diazi and Sylvilagus floridanus. We describe for the first time the partial repertoires of T2R genes for Lagomorphs species, other than the common rabbit. Our phylogenetic analyses indicate that sequence proximity levels follow the established taxonomic classification

Topics: Oryctolagus, TAS2R, lagomorph, Lepus, Sylvilagus, Romerolagus, Genetics, QH426-470
Publisher: Frontiers Media S.A.
Year: 2016
DOI identifier: 10.3389/fgene.2016.00055
OAI identifier: oai:doaj.org/article:4778ca007a134368977a7bad57a7bd82
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/1664-8021 (external link)
  • http://journal.frontiersin.org... (external link)
  • https://doaj.org/article/4778c... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.