Location of Repository

Investigating evolutionary conservation of dendritic cell subset identity and functions

By Thien-Phong eVu Manh, Nicolas eBertho, Anne eHosmalin, Isabelle eSchwartz-Cornil and Marc eDALOD

Abstract

Dendritic cells (DC) were initially defined as mononuclear phagocytes with a dendritic morphology and an exquisite efficiency for naïve T cell activation. DC encompass several subsets initially identified by their expression of specific cell surface molecules and later shown to excel in distinct functions and to develop under the instruction of different transcription factors or cytokines. Very few cell surface molecules are expressed in a specific manner on any immune cell type. Hence, to identify cell types, the sole use of a small number of cell surface markers in classical flow cytometry can be deceiving. Moreover, the markers currently used to define mononuclear phagocyte subsets vary depending on the tissue and animal species studied and even between laboratories. This has led to confusion in the definition of DC subset identity and in their attribution of specific functions. There is a strong need to identify a rigorous and consensus way to define mononuclear phagocyte subsets, with precise guidelines potentially applicable throughout tissues and species. We will discuss the advantages, drawbacks and complementarities of different methodologies: cell surface phenotyping, ontogeny, functional characterization and molecular profiling. We will advocate that gene expression profiling is a very rigorous, largely unbiased and accessible method to define the identity of mononuclear phagocyte subsets, which strengthens and refines surface phenotyping. It is uniquely powerful to yield new, experimentally testable, hypotheses on the ontogeny or functions of mononuclear phagocyte subsets, their molecular regulation and their evolutionary conservation. We propose defining cell populations based on a combination of cell surface phenotyping, expression analysis of hallmark genes and robust functional assays, in order to reach a consensus and integrate faster the huge but scattered knowledge accumulated by different laboratories on different cell types, organs and species

Topics: Dendritic Cells, Sheep, Vaccination, human, Mouse, Comparative genomics, Immunologic diseases. Allergy, RC581-607
Publisher: Frontiers Media S.A.
Year: 2015
DOI identifier: 10.3389/fimmu.2015.00260
OAI identifier: oai:doaj.org/article:1b03ad4315d04ae497697efea10c6187
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/1664-3224 (external link)
  • http://journal.frontiersin.org... (external link)
  • https://doaj.org/article/1b03a... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.