Skip to main content
Article thumbnail
Location of Repository

Metabolic functions of Pseudomonas fluorescens strains from Populus deltoides depend on rhizosphere or endosphere isolation compartment

By Collin M Timm, Alisha G Campbell, Sagar M Utturkar, Sagar M Utturkar, Se Ran eJun, Rebecca E Parales, Watumesa eTan, Michael Scott Robeson, Tse-Yuan eLu, Sara eJawdy, Steven D. Brown, Steven D. Brown, David W Ussery, Christopher Warren Schadt, Christopher Warren Schadt, Gerald A Tuskan, Mitchel J Doktycz, David Joseph Weston and Dale Adam Pelletier

Abstract

The bacterial microbiota of plants is diverse, with 1,000s of operational taxonomic units (OTUs) associated with any individual plant. In this work we investigate the differences between 19 sequenced Pseudomonas fluorescens strains, isolated from Populus deltoides rhizosphere and endosphere and which represent a single OTU, using phenotypic analysis, comparative genomics, and metabolic models. While no traits were exclusive to either endosphere or rhizosphere P. fluorescens isolates, multiple pathways relevant for plant-bacterial interactions are enriched in endosphere isolate genomes. Further, growth phenotypes such as phosphate solubilization, protease activity, denitrification and root growth promotion are biased towards endosphere isolates. Endosphere isolates have significantly more metabolic pathways for plant signaling compounds and an increased metabolic range that includes utilization of energy rich nucleotides and sugars, consistent with endosphere colonization. Rhizosphere P. fluorescens have fewer pathways representative of plant-bacterial interactions but show metabolic bias towards chemical substrates often found in root exudates. This work reveals the diverse functions that may contribute to colonization of the endosphere by bacteria and are enriched among closely related isolates

Topics: Metabolism, Populus, microbiome, metabolic modeling, rhizosphere, endosphere, Microbiology, QR1-502
Publisher: Frontiers Media S.A.
Year: 2015
DOI identifier: 10.3389/fmicb.2015.01118
OAI identifier: oai:doaj.org/article:83c3d5cf10e546fd90a28c53cd5a1b2e
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/1664-302X (external link)
  • http://journal.frontiersin.org... (external link)
  • https://doaj.org/article/83c3d... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.