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Evaluation on the Effectiveness of 2-Deoxyglucose-6-phosphate phosphatase (DOGR1) Gene as a Selectable Marker for Oil Palm (Elaeis guineensis Jacq.) Embryogenic Calli Transformation Mediated by Agrobacterium tumefaciens.

By Abang Masli eDayang Izawati, Mat Yunus eAbdul Masani, Ismanizan eIsmail and Ahmad Parveez eGhulam Kadir

Abstract

DOGR1, which encodes for 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOGR1 gene, was transformed into oil palm embryogenic calli mediated by Agrobacterium tumefaciens strain LBA4404. Transformed embryogenic calli were exposed to 400 mg l–1 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOGR1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOGR1 gene and 2-DOG for regenerating transgenic oil palm

Topics: Agrobacterium-mediated transformation, Oil palm, 2-deoxyglucose, Embryogenic calli, DOGR1 gene, Plant culture, SB1-1110
Publisher: Frontiers Media S.A.
Year: 2015
DOI identifier: 10.3389/fpls.2015.00727
OAI identifier: oai:doaj.org/article:8ed4844ed614412091c2c35b6cf2b806
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