Location of Repository

DISTRIBUTION OF DNA DAMAGE REPAIR GENE POLYMORPHISM hOGG1, XRCC1 and p53 AMONG SICKLE CELL DISEASE PATIENTS IN INDIA

By Sudhansu Sekhar Nishank

Abstract

Background– Defect in DNA damage repair genes due to oxidative stress predispose the humans to malignancies. There are many cases of association of malignancies with sickle cell disease patients (SCD) throughout the world, the molecular cause of which has never been investigated. DNA damage repair genes such as  hOGG1, XRCC1 and p53 play significant role in repair of DNA damage during oxidative stress but the distribution and clinical effect of these genes are not known till date in SCD patients who are associated with oxidative stress related clinical complications.         Objective – The aim of the study was to characterize the distribution and clinical effect of DNA damage gene polymorphisms p53 (codon 72 Arg> Pro), hOGG1 (codon 326 Ser>Cyst) and XRCC1 (codons 194 Arg>Trp, codon 280 Arg> His, codon 399 Arg> Gln) among SCD patients of  central India.  Methods- A case control study of  250 SCD patients and 250 normal individuals were investigated by PCR-RFLP techniques.      Result- The prevalence of mutant alleles of hOGG1 gene, XRCC1 codon 280 Arg>His  were found to be significantly high among SCD patients as compared to controls. However, SCD patients did not show clinical association with any of these DNA repair gene polymorphisms.   Conclusion- This indicates that hOGG1, p53  and XRCC1 gene polymorphisms  may not have any clinical impact among SCD patients in India

Topics: hOGG1 gene, p53 gene, XRCC1 gene, sickle cell disease, malignancies, Diseases of the blood and blood-forming organs, RC633-647.5
Publisher: PAGEPress Publications
Year: 2015
DOI identifier: 10.4084/mjhid.2015.046
OAI identifier: oai:doaj.org/article:441ca1b7f65044acbc5b1d3b8b5c4f5c
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/2035-3006 (external link)
  • http://www.mjhid.org/index.php... (external link)
  • https://doaj.org/article/441ca... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.