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FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS

By A. A. Grazhdantseva, G. F. Sivolobova, M. Major, E. V. Chub, A. N. Shvalov, P. V. Yudin, S. V. Netesov, V. B. Loktev and G. V. Kochneva

Abstract

<span>Antigenic profiles of envelope glycoproteins of hepatitis C virus presented by three genotypes 1b, 2a/2c and 3a, which are most widespread in the territory of Russia and, in particular, in Novosibirsk, were studied using a panel of overlapping synthetic peptides. It was shown that highly immunogenic peptide epitopes of Е1 and Е2 proteins common for all HCV genotypes, are located in amino acid positions 250-260, 315-325 (Е1 protein), 390-400 (hypervariable region 1), 430-440, and 680-690 (Е2 protein). The greatest inter-genotypic differences were recorded in positions 280-290, 410-430 and 520-540. A novel antigenic determinant was detected in the region of aa 280-290 of the Е1 protein which was typical only for HCV 2a/2c genotype. A broad variation in the boundaries for the most epitopes suggests a high variability of the Е1 and Е2 viral proteins; however, a similar repertoire of antibodies induced by different HCV genotypes indicates to an opportunity of designing a new generation of cross-reactive HCV vaccines based on mapping of the E1 and E2 antigenic regions.</span

Topics: вирус гепатита С, генотип, пептидный анализ, антигенная детерминанта, Immunologic diseases. Allergy, RC581-607
Publisher: SPb RAACI
Year: 2010
OAI identifier: oai:doaj.org/article:088055b801fe4031bb57269c7a4ab8c1
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