Skip to main content
Article thumbnail
Location of Repository

FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS

By A. A. Grazhdantseva, G. F. Sivolobova, M. Major, E. V. Chub, A. N. Shvalov, P. V. Yudin, S. V. Netesov, V. B. Loktev and G. V. Kochneva

Abstract

Antigenic profiles of envelope glycoproteins of hepatitis C virus presented by three genotypes 1b, 2a/2c and 3a, which are most widespread in the territory of Russia and, in particular, in Novosibirsk, were studied using a panel of overlapping synthetic peptides. It was shown that highly immunogenic peptide epitopes of Е1 and Е2 proteins common for all HCV genotypes, are located in amino acid positions 250-260, 315-325 (Е1 protein), 390-400 (hypervariable region 1), 430-440, and 680-690 (Е2 protein). The greatest inter-genotypic differences were recorded in positions 280-290, 410-430 and 520-540. A novel antigenic determinant was detected in the region of aa 280-290 of the Е1 protein which was typical only for HCV 2a/2c genotype. A broad variation in the boundaries for the most epitopes suggests a high variability of the Е1 and Е2 viral proteins; however, a similar repertoire of antibodies induced by different HCV genotypes indicates to an opportunity of designing a new generation of cross-reactive HCV vaccines based on mapping of the E1 and E2 antigenic regions

Topics: hepatitis C virus, genоtype, peptide analysis, antigenic determinant, Immunologic diseases. Allergy, RC581-607
Publisher: SPb RAACI
Year: 2010
OAI identifier: oai:doaj.org/article:088055b801fe4031bb57269c7a4ab8c1
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/2313-741X (external link)
  • https://doaj.org/toc/1563-0625 (external link)
  • http://mimmun.ru/mimmun/articl... (external link)
  • https://doaj.org/article/08805... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.