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EXPRESSION OF PATTERN-RECOGNIZING RECEPTORS AND TRANSCRIPTIONAL REGULATORS OF T HELPER CELL DIFFERENTIATION BY INTESTINAL LYMPHOCYTES IN EXPERIMENTAL ILEITIS AND UPON ADMINISTRATION OF SIMVASTATIN AND INTERLEUKIN-1 RECEPTOR ANTAGONIST

By A. S. Zherebiatiev, A. M. Kamyshnyi and V. A. Kamyshnaya

Abstract

<p>Pathogenesis of inflammatory bowel disease is complicated and multifactorial. T helper cells represent components of adaptive immune response, whereas Toll-like receptors, NOD-likereceptors, RIG-I-like receptors are involved in maintenance of mucosal, as well as commensal homeostasis. Aim of study was to evaluate the ability of Simvastatin and IL-1 receptor antagonist to modify the course of experimental ileitis in rats, with a focus on expression of TLR2, TLR4, NOD2, RIG-I, like as T-bet, GATA-3, RORγt, and FoxP3 transcription factors by resident lymphocytes in the small intestine. </p><p>Experiments were carried out with male Wistar rats (5 to 7 months old). The immunopositive lymphocytes were determined by means of direct and indirect immunofluorescence technique, using monoclonal rat antibodies. </p><p>Development of acute and chronic ileitis was associated with unidirectional tendency for increase of TLR2<span>+ </span>lymphocyte numbers, and decrease in total TLR4<span>+ </span>and FoxP3<span>+ </span>lymphocyte counts in lymphoid structures of ileum. Treatment of experimental animals with Simvastatin and ARIL-1 during the development of experimental pathology was accompanied by decrease of RORγt<span>+ </span>and T-bet<span>+ </span>lymphocytes, along with increasing total numbers of FoxP3<span>+ </span>lymphocytes. </p><p>Simvastatin and antagonist of IL-1 receptors seem to exert a beneficial effect upon the course and outcomes in the indomethacin-induced rat ileitis model, via changing expression of pattern-recognizing receptors on lymphocytes and modulation of balance between different T helper cell subsets of intestinal tissues.</p

Topics: илеит, образ-распознающие рецепторы, антагонист рецепторов интерлейкина-1, симвастатин, Immunologic diseases. Allergy, RC581-607
Publisher: SPb RAACI
Year: 2015
OAI identifier: oai:doaj.org/article:75c175b603c14cd481f0bc9dcb3bda3f
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