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AMBIGUITY OF LOCI DURING HLA-TYPING ON SSO TECHNOLOGY AND ATTEMPT TO RESOLVE THEM

By M. A. Loginova, I. V. Paramonov and N. P. Trofimova

Abstract

<div class="page" title="Page 1"><div class="section"><div class="layoutArea"><div class="column"><p><span>Sequence speci</span><span>fi</span><span>c oligonucleotides typing was used to identify human leukocyte antigen (HLA)-A, B, DRB1 alleles from 705 recruited volunteers with Volga Federal District for unrelated hematopoietic stem cell registry and 155 of their number at locus HLA-C. 48 samples cannot be entered into the database because of ambiguities in the identi</span><span>fi</span><span>cation of allelic loci on HLA-class I – HLA-A, HLA-B. To resolution of ambiguity use reagents kits AlleleSEQR HLA Sequencing, which allowed to reveal the ambiguity of the locus HLA-A, 44% of cases, the loci HLA-B and HLA-C – 40% of cases. Application software HARPs Finder showed the possibility of resolution of all identi</span><span>fi</span><span>ed allelic ambiguities (with the exception of types of ambiguity – A*03/A*32 or A*74:13/A*32:04 and A*01/A*11 or A*36:04/A*11) with the addition of basic kits AlleleSEQR HLA Sequencing reagents kit Al- leleSEQR HARPs. </span></p></div></div></div></div

Topics: HLA-типирование., Surgery, RD1-811
Publisher: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
Year: 2010
OAI identifier: oai:doaj.org/article:33b006c5f7734b86ae58e674056981db
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