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Increased Yield of Biotransformation of Androsta-1, 4-Dien-3, 17-Dione from Β-Sitosterol by Using Sulfobutyl Ether-Β-Cyclodextrin Complexation Technique

By Wang Jingwen, Guan Shimin, Cai Baoguo, Zhang Shuo, Yang Shulin, Zhou Yue, Rong Shaofeng and Li Qianqian

Abstract

Substrate solubility in steroid biotransformation is critical for enhancing the biotransformation of hydrophobic compounds. In this study, the sulfobutyl ether-β-cyclodextrin (SBE-β-CD) complexation technique was used for the biotransformation of β-sitosterol to androsta-1, 4-diene-3, 17-dione with Mycobacterium ATCC25795. The production yield was increased by 26.72%, and the biotransformation course was shortened by 24h using β-sitosterol/SBE-β-CD inclusion complexes as substrates (1.0 g/L). Fourier transform infrared spectroscopy and differential scanning calorimetry indicated that an inclusion complex was formed between SBE-β-CD and β-sitosterol. The complex significantly increased the solubility of β-sitosterol and improved the biotransformation efficiency of the substrate

Topics: β-sitosterol, biotransformation, Mycobacterium, androsta-1, 4-dien-3, 17-dione, Engineering (General). Civil engineering (General), TA1-2040
Publisher: EDP Sciences
Year: 2016
DOI identifier: 10.1051/matecconf
OAI identifier: oai:doaj.org/article:e1a7f29d3d6c46e8afc1407df34cb873
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