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Purification, characterization and antimicrobial activity of chitinase from marine-derived Aspergillus terreus

By Aida M. Farag, Hanan M. Abd-Elnabey, Hassan A.H. Ibrahim and Moustafa El-Shenawy

Abstract

Chitinase (EC 3.2.1.14) was produced from the culture filtrate of marine-derived Aspergillus terreus and purified by 65% ammonium sulphate precipitation, followed by gel filtration on Sephadex G-100 and DEAE-Sephadex A-50 ion exchange chromatography, with 5.16-fold of purification and specific activity of 182.08 U/mg protein. The molecular weight of the purified chitinase was 60 kDa, determined by a sodium dodecyl sulphate polyacrylamide gel electrophoresis. The optimum pH and temperature of purified chitinase were 5.6 and 50 °C, respectively. The chitinase enzyme was stable from pH 5 to 7.5 and stable up to 70 °C. The effect of activators and inhibitors was studied, Hg+, pb, EDTA, ethanol, methanol and acetone strongly inhibited the enzyme activity, while, metal ions such as Ca2+, Mn2+ and Na2+ highly increased chitinase activity. The purified chitinase produced by A. terreus inhibited the growth of Aspergillus niger, Aspergillus oryzae, Penicillum oxysporium, Rhizocotonia solani, Candida albicans and Fusarium solani, while did not inhibit the growth of Rhizopus oryzae. Moreover, the purified enzyme had antibacterial effects against some pathogenic bacteria such as; Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa, while, it had not any activity against Escherichia coli, Aeromonas hydrophila and Photobacterium damsela

Topics: Chitinase, Purification, Characterization, Aspergillus terreus, Antimicrobial activity, Aquaculture. Fisheries. Angling, SH1-691, Environmental sciences, GE1-350
Publisher: Elsevier
Year: 2016
DOI identifier: 10.1016/j.ejar.2016.04.004
OAI identifier: oai:doaj.org/article:3d26c64c59f84826a1ec9950bf3c086f
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