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Inclusion of a pH‑responsive amino acid‑based amphiphile in methotrexate‑loaded chitosan nanoparticles as a delivery strategy in cancer therapy

By Daniele R. Nogueira, Laís E. Scheeren, Letícia B. Macedo, Ana Isa P. Marcolino, Montserrat Mitjans Arnal, Ma. Pilar Vinardell Martínez-Hidalgo, Ma. Rosa Infante Martínez-Pardo, Ammad A. Farooqi and Clarice M. B. Rolim

Abstract

The encapsulation of antitumor drugs in nanosized systems with pH-sensitive behavior is a promising approach that may enhance the success of chemotherapy in many cancers. The nanocarrier dependence on pH might trigger an efficient delivery of the encapsulated drug both in the acidic extracellular environment of tumors and, especially, in the intracellular compartments through disruption of endosomal membrane. In this context, here we reported the preparation of chitosan-based nanoparticles encapsulating methotrexate as a model drug (MTX-CS-NPs), which comprises the incorporation of an amino acid-based amphiphile with pH-responsive properties (77KS) on the ionotropic complexation process. The presence of 77KS clearly gives a pH-sensitive behavior to NPs, which allowed accelerated release of MTX with decreasing pH as well as pH-dependent membrane-lytic activity. This latter performance demonstrates the potential of these NPs to facilitate cytosolic delivery of endocytosed materials. Outstandingly the cytotoxicity of MTX-loaded CS-NPs was higher than free drug to MCF-7 tumor cells and, to a lesser extent, to HeLa cells. Based on the overall results, MTX-CS-NPs modified with the pH-sensitive surfactant 77KS could be potentially useful as a carrier system for intracellular drug delivery and, thus, a promising targeting anticancer chemotherapeutic agent

Topics: Quitosan, Nanopartícules, Agents tensioactius, Metotrexat, Medicaments antineoplàstics, Chitosan, Nanoparticles, Surface active agents, Methotrexate, Antineoplastic agents
Publisher: Springer Verlag
Year: 2016
DOI identifier: 10.1007/s00726-015-2075-1
OAI identifier: oai:diposit.ub.edu:2445/97360
Journal:

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