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Use of a proposed antimicrobial susceptibility testing method for Haemophilus parasuis

By Denise Ann E. Dayao, Marco Kienzle, Justine S. Gibson, Patrick J. Blackall and Conny Turni

Abstract

The aim of this study was to examine the antimicrobial susceptibility of 97 Haemophilus parasuis cultured from Australian pigs. As there is no existing standard antimicrobial susceptibility technique available for H. parasuis, methods utilising the supplemented media, BA/SN for disc diffusion and test medium broth (TMB) for a microdilution technique, were initially evaluated with the reference strains recommended by the Clinical and Laboratory Standards Institute. The results of the media evaluation suggested that BA/SN and TMB can be used as suitable media for susceptibility testing of H. parasuis. The proposed microdilution technique was then used with 97 H. parasuis isolates and nine antimicrobial agents. The study found that Australian isolates showed elevated minimum inhibitory concentrations (MICs) for ampicillin (1%), penicillin (2%), erythromycin (7%), tulathromycin (9%), tilmicosin (22%), tetracycline (31%) and trimethoprim-sulfamethoxazole (40%). This study has described potential antimicrobial susceptibility methods for H. parasuis and has detected a low percentage of Australian H. parasuis isolates with elevated antimicrobial MICs

Topics: Antimicrobial susceptibility testing, BA/SN, Glässer\u27s disease, Haemophilus parasuis, Test medium broth (TMB), 3400 Veterinary, 2404 Microbiology
Publisher: Elsevier
Year: 2014
DOI identifier: 10.1016/j.vetmic.2014.06.010
OAI identifier: oai:espace.library.uq.edu.au:UQ:336857

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