Adrenergic \xce\xb22 receptor activation stimulates anti-inflammatory properties of dendritic cells in vitro

Abstract

Vagal nerve efferent activation has been shown to ameliorate the course of many inflammatory disease states. This neuromodulatory effect has been suggested to rest on acetylcholine receptor (AChR) activation on tissue macrophages or dendritic cells (DCs). In more recent studies, vagal anti-inflammatory activity was shown involve adrenergic, splenic, pathways. Here we provide evidence that the adrenergic, rather than cholinergic, receptor activation on bone marrow derived DCs results in enhanced endocytosis uptake, enhanced IL-10 production but a decreased IL-6, IL-12p70 and IL-23 production. In antigen specific T cell stimulation assays, adrenergic \xce\xb22 receptor activation on bone marrow DCs led to an enhanced potential to induce Foxp3 positive suppressive Treg cells. These effects were independent of IL10-R activation, TGF\xce\xb2 release, or retinoic acid (RA) secretion. Hence, adrenergic receptor \xce\xb22 activation modulates DC function resulting in skewing towards anti-inflammatory T cell phenotypes