Skip to main content
Article thumbnail
Location of Repository

Functional annotation of proteomic data from chicken heterophils and macrophages induced by carbon nanotube exposure

By Yun-Ze Li, Chung-Shi Cheng, Chao-Jung Chen, Zi-Lin Li, Yao-Tung Lin, Shuen-Ei Chen and San-Yuan Huang


With the expanding applications of carbon nanotubes (CNT) in biomedicine and agriculture, questions about the toxicity and biocompatibility of CNT in humans and domestic animals are becoming matters of serious concern. This study used proteomic methods to profile gene expression in chicken macrophages and heterophils in response to CNT exposure. Two-dimensional gel electrophoresis identified 12 proteins in macrophages and 15 in heterophils, with differential expression patterns in response to CNT co-incubation (0, 1, 10, and 100 µg/mL of CNT for 6 h) (p < 0.05). Gene ontology analysis showed that most of the differentially expressed proteins are associated with protein interactions, cellular metabolic processes, and cell mobility, suggesting activation of innate immune functions. Western blot analysis with heat shock protein 70, high mobility group protein, and peptidylprolyl isomerase A confirmed the alterations of the profiled proteins. The functional annotations were further confirmed by effective cell migration, promoted interleukin-1β secretion, and more cell death in both macrophages and heterophils exposed to CNT (p < 0.05). In conclusion, results of this study suggest that CNT exposure affects protein expression, leading to activation of macrophages and heterophils, resulting in altered cytoskeleton remodeling, cell migration, and cytokine production, and thereby mediates tissue immune responses

Topics: Animals, Cell Movement, Cell Survival, Cells, Cultured, Chickens, Gene Expression Regulation, Gene Ontology, Interleukin-1beta, Macrophages, Nanotubes, Carbon, Proteomics
Year: 2015
DOI identifier: 10.3390/ijms15058372
OAI identifier:

Suggested articles

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.