Skip to main content
Article thumbnail
Location of Repository

The NAC-like gene ANTHER INDEHISCENCE FACTOR acts as a repressor that controls anther dehiscence by regulating genes in the jasmonate biosynthesis pathway in Arabidopsis

By C.-F. Shih, W.-H. Hsu, Y.-J. Peng and C.-H. Yang

Abstract

ANTHER INDEHISCENCE FACTOR (AIF), a NAC-like gene, was identified in Arabidopsis. In AIF:GUS flowers, β-glucuronidase (GUS) activity was detected in the anther, the upper parts of the filaments, and in the pollen of stage 7-9 young flower buds; GUS activity was reduced in mature flowers. Yellow fluorescent protein (YFP)+AIF-C fusion proteins, which lacked a transmembrane domain, accumulated in the nuclei of the Arabidopsis cells, whereas the YFP+AIF fusion proteins accumulated in the membrane and were absent in the nuclei. Further detection of a cleaved AIF protein in flowers revealed that AIF needs to be processed and released from the endoplasmic reticulum in order to function. The ectopic expression of AIF-C caused a male-sterile phenotype with indehiscent anthers throughout flower development in Arabidopsis. The presence of a repressor domain in AIF and the similar phenotype of indehiscent anthers in AIF-C+SRDX plants suggest that AIF acts as a repressor. The defect in anther dehiscence was due to the down-regulation of genes that participate in jasmonic acid (JA) biosynthesis, such as DAD1/AOS/AOC3/OPR3/OPCL1. The external application of JA rescued the anther indehiscence in AIF-C and AIF-C+SRDX flowers. In AIF-C+VP16 plants, which are transgenic dominant-negative mutants in which AIF is converted to a potent activator via fusion to a VP16-AD motif, the anther dehiscence was promoted, and the expression of DAD1/AOS/AOC3/OPR3/OPCL1 was up-regulated. Furthermore, the suppression of AIF through an antisense strategy resulted in a mutant phenotype similar to that observed in the AIF-C+VP16 flowers. The present data suggest a role for AIF in controlling anther dehiscence by suppressing the expression of JA biosynthesis genes in Arabidopsis

Topics: ANTHER INDEHISCENCE FACTOR, Anther dehiscence, NAC-like gene, jasmonate signalling, repressor., Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, Biosynthetic Pathways, Cyclopentanes, DNA, Complementary, Down-Regulation, Endoplasmic Reticulum, Flowers, Genes, Dominant, Genes, Plant, Glucuronidase, Models, Biological, Molecular Sequence Data, Mutation, Oxylipins, Phenotype, Plant Infertility, Plants, Genetically Modified, Pollen, Protein Transport, Protoplasts, Repressor Proteins, Up-Regulation, Gene Expression Regulation, Plant
Year: 2015
DOI identifier: 10.1093/jxb
OAI identifier: oai:ir.lib.nchu.edu.tw:11455/86531
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://hdl.handle.net/11455/86... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.