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AtRH57, a DEAD-box RNA helicase, is involved in feedback inhibition of glucose-mediated abscisic acid accumulation during seedling development and additively affects pre-ribosomal RNA processing with high glucose

By Yi-Feng Hsu, Yun-Chu Chen, Yu-Chun Hsiao, Bing-Jyun Wang, Shih-Yun Lin, Wan-Hsing Cheng, Guang-Yuh Jauh, John J. Harada and Co-Shine Wang


The Arabidopsis thaliana T-DNA insertion mutant rh57-1 exhibited hypersensitivity to glucose (Glc) and abscisic acid (ABA). The other two rh57 mutants also showed Glc hypersensitivity similar to rh57-1, strongly suggesting that the Glc-hypersensitive feature of these mutants results from mutation of AtRH57. rh57-1 and rh57-3 displayed severely impaired seedling growth when grown in Glc concentrations higher than 3%. The gene, AtRH57 (At3g09720), was expressed in all Arabidopsis organs and its transcript was significantly induced by ABA, high Glc and salt. The new AtRH57 belongs to class II DEAD-box RNA helicase gene family. Transient expression of AtRH57-EGFP (enhanced green fluorescent protein) in onion cells indicated that AtRH57 was localized in the nucleus and nucleolus. Purified AtRH57-His protein was shown to unwind double-stranded RNA independent of ATP in vitro. The ABA biosynthesis inhibitor fluridone profoundly redeemed seedling growth arrest mediated by sugar. rh57-1 showed increased ABA levels when exposed to high Glc. Quantitative real time polymerase chain reaction analysis showed that AtRH57 acts in a signaling network downstream of HXK1. A feedback inhibition of ABA accumulation mediated by AtRH57 exists within the sugar-mediated ABA signaling. AtRH57 mutation and high Glc conditions additively caused a severe defect in small ribosomal subunit formation. The accumulation of abnormal pre-rRNA and resistance to protein synthesis-related antibiotics were observed in rh57 mutants and in the wild-type Col-0 under high Glc conditions. These results suggested that AtRH57 plays an important role in rRNA biogenesis in Arabidopsis and participates in response to sugar involving Glc- and ABA signaling during germination and seedling growth

Topics: Arabidopsis thaliana seeds, RNA helicase, abscisic acid, glucose-hypersensitive, rRNA biogenesis, seedling growth, Abscisic Acid, Amino Acid Motifs, Arabidopsis, Arabidopsis Proteins, DEAD-box RNA Helicases, Gene Expression, Gene Expression Regulation, Plant, Germination, Glucose, Models, Biological, Mutagenesis, Insertional, Phenotype, Plant Growth Regulators, Plants, Genetically Modified, RNA Precursors, RNA, Ribosomal, Ribosomes, Seedling, Seeds, Signal Transduction
Year: 2015
DOI identifier: 10.1111/tpj.12371
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