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Nanostructured electrochemical biosensor for th0065 detection of the weak binding between the dengue virus and the CLEC5A receptor

By Yen-Ting Tung, Ming-Fang Wu, Gou-Jen Wang and Shie-Liang Hsieh


In this paper, we develop an effective method for detecting weak molecular bonding between the dengue virus (DV) and its receptor C-type lectin domain family 5, member A (CLEC5A). The CLEC5A-DV interaction is critical for DV-induced hemorrhagic fever and shock syndrome, so the sensing of CLEC5A-DV binding is crucial to realize a thorough study of the pathogenesis of dengue fever. Through a highly sensitive nanostructured sensing electrode of gold nanoparticles (GNPs) uniformly deposited on a nanohemisphere array, a label-free detection of the ultra weak binding between CLEC5A and the DV can be performed with electrochemical impedance spectroscopy (EIS). Experimental results demonstrate that the proposed approach is a highly promising method for investigating weak molecular interactions such as the ligand-receptor interaction of dengue fever, enterovirus (EV), or the interaction between cancer surface glycoproteins and their receptors

Topics: Anodic aluminum oxide, CLEC5A, Dengue virus, Electrochemical impedance spectroscopy, Nanostructured biosensor, Biosensing Techniques, Dengue, Dengue Virus, Dielectric Spectroscopy, Electrodes, Equipment Design, Gold, Humans, Lectins, C-Type, Nanostructures, Protein Binding, Receptors, Cell Surface
Year: 2015
DOI identifier: 10.1016/j.nano.2014.03.009
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