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Lemon protein disulfide isomerase: cDNA cloning and biochemical characterization

By Yu-Ting Chen, Lisa Wen, Kuo-Chuan Ho, Rong-Huay Juang and Chi-Tsai Lin

Abstract

BackgroundProtein disulfide isomerases (PDIs), a family of structurally related enzymes, aid in protein folding by catalyzing disulfide bonds formation, breakage, or isomerization in newly synthesized proteins and thus.ResultsA ClPDI cDNA (1828 bp, GenBank accession HM641784) encoding a putative PDI from Citrus limonum was cloned by polymerase chain reaction (PCR). The DNA sequence encodes a protein of 500 amino acids with a calculated molecular mass of 60.5 kDa. The deduced amino acid sequence is conserved among the reported PDIs. A 3-D structural model of the ClPDI has been created based on the known crystal structure of Homo sapiens (PDB ID: 3F8U_A). The enzyme has two putative active sites comprising the redox-active disulfides between residues 60–63 and 405–408 (motif CGHC). To further characterize the ClPDI, the coding region was subcloned into an expression vector pET-20b (+), transformed into E. coli Rosetta (DE3)pLysS, and recombinant protein expressed. The recombinant ClPDI was purified by a nickel Sepharose column. PDI’s activity was assayed based on the ability of the enzyme to isomerize scrambled RNase A (sRNase A) to active enzyme. The KM, kcat and kcat/KM values were 8.3 × 10-3 μM, 3.0 × 10-5 min-1, and 3.6 × 10-1 min-1 mM-1. The enzyme was most active at pH 8.ConclusionsThe advantage of this enzyme over the PDI from all other sources is its low KM. The potential applications of this PDI in health and beauty may worth pursuing

Topics: Citrus limonum, Protein disulfide isomerase (PDI), Scrambled RNase A (sRNase A), Three-dimension structural model (3-D structural model)
Year: 2014
DOI identifier: 10.1186/1999-3110-54-34
OAI identifier: oai:ir.lib.nchu.edu.tw:11455/85152
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