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Accelerated colorimetric immunosensingusing surface-modified porous monolithsand gold nanoparticles

By Shao-Hsuan Chuag, Guan-Hua Chen, Hsin-Hao Chou, Shu-Wei Shen and Chien-Fu Chen

Abstract

A rapid and sensitive immunoassay platform integrating polymerized monoliths and gold nanoparticles (AuNPs) has been developed. The porous monoliths are photopolymerized in situ within a silica capillary and serve as solid support for high-mass transport and high-density capture antibody immobilization to create a shorter diffusion length for antibody–antigen interactions, resulting in a rapid assay and low reagent consumption. AuNPs are modified with detection antibodies and are utilized as signals for colorimetric immunoassays without the need for enzyme, substrate and sophisticated equipment for quantitative measurements. This platform has been verified by performing a human IgG sandwich immunoassay with a detection limit of 0.1 ng ml−1. In addition, a single assay can be completed in 1 h, which is more efficient than traditional immunoassays that require several hours to complete

Year: 2014
DOI identifier: 10.1088/1468-6996
OAI identifier: oai:ir.lib.nchu.edu.tw:11455/84973

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