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Reduction of incubation time and enhancement of analyte adhesion uniformity of impedance biosensors using microvibration method

By Jaw-Ji Tsai, Yi-Fen Liu, En-Chih Liao, Jau-Liang Chen and Gou-Jen Wang


Electrochemical impedance spectroscopy (EIS) analysis can detect an analyte with high sensitivity inshort time. The self-assembled monolayer (SAM) technique is usually used for attaching analytes to atransducer. In general, the relatively time-consuming incubation time and the non-uniform immobilizationof an analyte on the sensing electrode are the commonly encountered problems. This study proposesa simple microvibration method based on Bernoulli’s theory for improving incubation time and enhancingthe adhesion uniformity of the analyte. The detection of the group-2 allergen Der p2 demonstratesthat a suitable applied frequency at a fixed amplitude can considerably reduce the sample preparationtime and improve the adhesion and adhesive uniformity of the analyte. It is also found that compared tothe conventional dipping method the microvibration method can give a relatively large detection range,provide a relatively better detection linearity, and result in a standard detection curve with a relativelylarge slope so that the sensitivity of the sensor can be increased and the detection error due to measurementvariations can be reduced. The proposed scheme can be further applied to other detection methodsthat use surface-bound reactions for analyte immobilization

Year: 2014
DOI identifier: 10.1016/j.snb.2013.01.007
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