In this study, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and phosphoribulokinase(PrkA) were overexpressed individually and in combination in Escherichia coli under different fermentationconditions. While wild-type E. coli produced 0.731 mol of CO2 per consumption of one mole of arabinose,engineered E. coli JB, containing both heterologous Rubisco and PrkA, produced only 0.621 mol ofCO2 per consumption of one mole of arabinose. This represents a 15% reduction in CO2 emission andachieves 38% of theoretical CO2 reduction. The CO2 fixation rate of Rubisco-based engineered E. coli JBis 67 mg-CO2 mole-arabinose 1 L 1 h 1, which is comparable to the performance of microalgae and cyanobacteria.It has been found that overexpressing Rubisco dramatically elevates the bacteria growth andsugar consumptions in the presence of oxygen and L-arabinose. It has been also found that overexpressingPrkA could demolish the balance of ATP regeneration, yet can be recovered simply by controlling thepH at 7.0 ± 0.1
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