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Determination of Viability in Bitter Gourd Pollen (Momordica charabtia L.).

By 張正桓, 宋妤, Cheng-huag Chang and Yu Sung


The viability of fore varieties of bitter gourd pollen was assessed with three histochemical methods﹐and by brewbaker and Kwack(1963)solution as growth medium.Bitter gourd pollen germination could be determined using B&K liquid growth medium containing \ud 10% sucrose﹐100 to 150 ppm H3BO3﹐200 to 300 ppm Ca(NO3)2﹐Ph7.0﹐and a culture temperature of 25 to 30℃.Among the histochemical methods﹐the FDA(fluorescein dicaetate)method was best for determining bitter gourd pollen viability. This method is easy and fast﹐and reqires the fewest observations﹐but it overestimates pollen viability.本研究以Brewbaker and Kwack(1963)液體培養基配方及三種組織化學(histochemical)法,檢定四品種苦瓜花粉活力的方法。'青皮'、'蘋果'、'大長'及'粉青'苦瓜花粉發芽以10%蔗糖、l00-150ppm的硼酸、200-300ppm的硝酸鈣之B&K液體培養基,將培養基pH調至7.0,並於25-30℃下培養為最適合的培養條件。FDA螢光法雖然有高估花粉活力之情形,但簡單快速又可處理大量重覆數樣品,適合用於苦瓜花粉活力檢定上

Topics: bitter gourd, pollen viability, in vitro germination, histochemical method, 苦瓜, 花粉活力, 體外發芽, 組織化學法
Year: 2014
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