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Effect of Support in the Growth of Phalaenopsis Seedlings in vitro

By 蔡媦婷, 朱建鏞, Wei-Ting Tsai and Chien-Young Chu


本研究以GA-7容器,培養基成分為Knudson C(1946)無機鹽類、蔗糖2OgL-1及馬鈴薯34gL-1,配合以不織布、微孔膜塑膠筏等支撐物進行蝴蝶蘭液體播種。當培養基量為50ml時,播於不織布或微孔膜塑膠筏者,經75天後有70%以上小植株產生水化。將播種75天之小植株,移植至固體培養基,經90天後,來自不織布培養之小植株水化率降至3%,且77%植株有正常葉片分化,與原來自固體培養者無差異。但苗來自微孔膜塑膠筏培養者僅20%之小植株可正常分化葉片。將液體培養基量降為40ml,並以不織布上舖棉紙為支撐物進行播種,經75天後小植株之水化百分率為3%,且小植株鮮重、乾重及乾/鮮重比值皆比固體培養者為高。將小植株繼代至不織布舖棉紙之液體培養45天後,其葉片較大、葉數亦較原為固體培養者多。In this study, non-woven fabric(L-N), filter paper(L-P)and microporous membrane raft(L-R)were used as supports in GA-7 vessels with liquid medium, containing Knudson C (1946)basal salts, 34 g L-1 potato and 20 g L-1 sucrose, for phalaenopsis seed culture. As 50 ml liquid medium was used per vessel, over 70% hyperhydric seedlings were observed after 75 days cultured in L-N or L-R. After transplanted to agar solidified medium for 90 days, hyperhydration rate of seedlings from L-N culture decreased to 3% and 70% of total seedlings developed into normal seedlings. As 40 ml liquid medium was used per vessel, hyperhydration rate was at 3% after 75 days of sowing as non-woven fabric covered with paper towel(L-NC)was used as support. The fresh and dry weight and of seedlings cultured on L-NC support were higher than that culture on solid medium. Seedlings from L-NC culture transplanted to liquid medium with L-NC support grew more leaves than that cultured on solid medium or cultured on liquid medium with vivid cube as support

Topics: Hyperhydration, Non-woven fabric, Microporous membrane raft, 不織布, 微孔膜塑膠筏, 水化
Year: 2014
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