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水稻子葉盤癒傷組織再生系統的建立

By 劉程煒, 曾夢蛟, Cheng-Wei Liu and Menq-Jiau Tseng

Abstract

本研究之目的為建立台灣栽培水稻品種之成熟胚誘導子葉盤癒傷組織的再生盤系。水稻成熟種子以無菌播種於含有2mg/L之2 ,4-D的N6基礎培養基10天後,置於全暗的環境下,子葉盤所誘導的癒傷組織分裂速度最快。添加2mg/L之kinetin與m/L 之NAA 的MS基礎培養基培養3週後,約52.7%的'台農67號'與98.9%的'台梗9號'水稻癒傷組織能夠分化出芽體。'台梗l號'與'台梗8號'於完全黑暗的環境下誘導的癒傷組織,移到STl培養基4週後,平均每顆癒傷組織能夠分化出8.8與7.8個綠芽體,在16小時明期與8小時暗期的環境中,平均綠芽體數則增加到12與15.2個。'台梗8號'於MSD培養基中16小時明期與8小時暗期的環境下3週後,於再生培養基27天後平均有11.2個綠芽體,'台農67號'則要全暗處理3週後,平均每顆癒傷組織能夠得到8.7個芽體。The purpose of this study is to develop a simple and highly efficient protocol for plant regeneration from scutella-derived embryogenic calli of rice (Oryza sativa L.). Vigorous growth of scutella-derived embryogenic calli was obtained from the mature rice seeds germinated in the N6 medium containing 2.0 mg/L of kinetin and 1.0 mg/L of napthaleneacetic acid for 10 days, and followed by cultivation in the dark for 10 days. Fifty-three and ninety-nine percentages of regeneration rate of green buds from embryogenic calli was achieved in the 'TN67' and 'TK9' rice, respectively, after three weeks of cultivationin the MS medium containing 2.0 mg/L of kinetin and 1.0 mg/L of napthaleneacetic acid. The average number of green buds emerged form each callus (GBP/callus ) was 8.8 and 7.8 in 'TK1' and 'TK8' rice, respectively, after four weeks of cultivation in the ST1 medium, while 12 and 15.2 GBP/callus, respectively, after supply with a 16-hr photoperiod. The GBP/callus was 11.2 in 'TK8' rice after four weeks of cultivation in the MSD medium supply with 16-hr photoperiod, but only 8.7 GBP/callus in 'TN67' rice after three weeks of cultivation in the dark

Topics: Rice, Scutellum, Callus, Regeneration, 水稻, 子葉盤, 癒傷組織, 再生
Year: 2014
OAI identifier: oai:ir.lib.nchu.edu.tw:11455/81741
Journal:

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