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he effect of regeneration of Pleurotus eryngii by adding nutrients

By 紀鴻淵 and Hung-Yuan Chi

Abstract

合作農場最主要以杏鮑菇的生產最大宗,但是杏鮑菇的太空包收割只有一次,每一包的收割約為180~250公克。一般菇類的採收如洋菇(Agaricus bisporus)、香菇(Lentinula edodes)、秀珍菇(Pleurotus ostreatus)都可以採收至少三次以上(Velazquez-Cedeno et al., 2002; Royse, 2001, Royse et al.,2008),而杏鮑菇只能採收一次,是因為第二週期的產量及品質不如第一週期,且出菇時間較長(彭,1997)。為了確定是養分或是水分流失造成的影響,筆者進行了補充養分以及水分的實驗,並觀察二次出菇的數量,且利用統計方法分析何種營養劑可以獲得最大的二次出菇數量。 牛奶和豆漿加水稀釋成1.25%、2.5%、5%;元素和海草粉加水稀釋成0.1%、0.2%、0.4%做為營養劑使用。由農場取已採收過一次菇之杏鮑菇太空包→編號→編組→利用抽籤方式隨機分配→置於栽培室→每週加入10ml之營養劑→採收→觀察記錄杏鮑菇之產量及品質。 實驗結果,杏鮑菇的採收重量、數量及生物轉換率很明顯的受到添加的營養劑不同而有所影響。不同濃度的牛奶液和元素液的添加對重量、數量及生物轉換率均有影響。不同濃度的豆漿液和元素液的添加則沒有太大影響。添加牛奶液2.5%、5%及豆漿液1.25%、2.5%和5%會得到較其他營養劑高的杏鮑菇採收重量,牛奶液2.5%會得到最高的重量;添加牛奶液2.5%、5%及豆漿液1.25%、5%會得到較其他營養劑高的杏鮑菇生物轉換率,牛奶液2.5%會得到最高的生物轉換率;添加牛奶液2.5%、5%及豆漿液1.25%、2.5%和5%會得到較其他營養劑高的杏鮑菇採收數量及平均大小,牛奶液2.5%會得到最高的數量,牛奶液5%會得到最高的平均大小。本實驗總採收的杏鮑菇數量共68個,重量分布主要集中在40-80公克之間,平均重量為62.3公克,依照目前市場上的分類,62.3公克會落在A級杏鮑菇的分類,屬於市場最好的價格。牛奶液2.5%及豆漿液5%有最高的產值。Pleurotus eryngii is the most production in the cooperative farm, but the bag typically harvest only one break and the harvest yield about 180-250 g per bag. Generally, the three breaks could be harvested by farmer in the general mushrooms like Agaricus bisporus, Lentinula edodes, and Pleurotus ostreatus (Velazquez-Cedeno et al., 2002; Royse, 2001,Royse et al., 2008). The reason for the only one harvest of Pleurotus eryngii is the yield and quality wasn’t better and the incubation time extended in the second time harvest (Peng, 1997). In order to confirm the effect of the lack of nutrients or water caused the second harvest difficultly, we designed an experiment to observe the harvest yield in the second time break by adding the nutrients and water. The different concentrations of 1.25%, 2.5%, 5% milk or soybean and 0.1%, 0.2%, 0.4% element or seaweed algae were added in the bags which were harvest one break. We chose the bags randomly and divided into 13 groups. The nutrients were added 10 ml per time in two week. The result, There were significant effects of the harvest yield, the harvest number and biological efficiency (BE) by adding the different nutrients. All of the different concentrations of milk or soybean caused the effects of the harvest yield, the harvest number and biological efficiency. Mushroom yields were significantly higher from adding 2.5%, 5% milk or 1.25%, 5% soybean were added than other nutrients, and the highest yield at 2.5% milk. The better BEs were harvested from adding 2.5%, 5% milk or 1.25%, 5% soybean, and the best BE at 2.5% milk. The more harvest number and average size were calculated from adding 2.5%, 5% milk or 1.25%, 2.5%, 5% soybean, and the most harvest number at 2.5% milk ; the most average size at 5% milk. In this experiment, the harvest number was a total of 68, and the weights were most distributed among 40-80 g. The average of weights was 62.3 g falling into the grade A of categories, and it was the best price in the market. The best benefit was earned from 2.5% milk and 5% soybean.第一章 前言........................................................................................................1 第二章 文獻回顧....................................................................................................2 第一節 真菌的介紹............................................................................................2 第二節 蠔菇屬介紹............................................................................................3 第三節 杏鮑菇之介紹........................................................................................4 一、 杏鮑菇之分類..........................................................................................4 二、 杏鮑菇之型態..........................................................................................4 三、 杏鮑菇之栽培..........................................................................................5 第三章 材料與方法................................................................................................8 第一節 合作農場之實地訪查............................................................................8 一、 訪查位置..................................................................................................8 二、 實驗時間.................................................................................................10 第二節 營養劑添加之實驗...............................................................................10 一、 太空包之製作.........................................................................................10 二、 杏鮑菇之栽培流程.................................................................................14 三、 實驗材料.................................................................................................19 四、 實驗流程.................................................................................................20 第四章 結果........................................................................................................24 一、 變異數分析(ANOVA).........................................................................24 二、 重量和生物轉換率...............................................................................25 三、 數量和平均大小...................................................................................30 四、 重量的分布...........................................................................................30 五、 碳及氮含量對杏鮑菇生長的影響.......................................................32 六、 營養劑添加後之預估產值...................................................................33 第五章 討論...........................................................................................................36 第六章 參考文獻...................................................................................................3

Topics: 論文, 中興大學, Thesis, National Chung Hsing University
Publisher: 生命科學院碩士在職專班
Year: 2014
OAI identifier: oai:ir.lib.nchu.edu.tw:11455/81156
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