The aim of this study was to survey Mycoplasma spp. infection in cats in central Taiwan, and to characterize Mycoplasma spp. by polymerase chain reaction (PCR). We collected 120 feline blood samples at National Chung-Hsing University Veterinary Medical Teaching Hospital and 5 veterinary hospitals from March to September 2011, and these samples were analyzed by PCR and blood examination. Of the 120 samples, 5 (4.2%) were Mycoplasma haemofelis (Mhf), 21 (17.5%) were Candidatus Mycoplasma haemominutum (CMhm), 2 (1.6%) cats were Candidatus Mycoplasma turicensis (CMt), and 3 (2.5%) were Candidatus Mycoplasma haemominutum and Mycoplasma haemofelis. The main symptoms of Mhf-infected cats included anemia, anorexia, and depression; and those of CMhm-infected cats included anemia and depression; and those of CMt-infected cats included anemia. In addition, we speculated that male, mixed breed and less than six-year-old cats were the risk factors for CMhm infection. The further 16S rRNA gene sequence analysis showed that the sequences of 5 Mhf Taiwan strains shared 100% identity with USA strain, and the sequences of 21 CMhm Taiwan strains shared 99.4-99.8% identity with United Kingdom and Israel strains, and the sequences of 2 CMt Taiwan strains shared 100% identity with Switzerland and South Africa strains. Phylogenetic analysis showed that Mhf, CMhm and CMt Taiwan strains were grouped with the same species. The data indicate that the main Mycoplasma spp. pathogens in cats in central Taiwan are CMhm, followed by Mhf and CMt.本研究目的為利用聚合酶連鎖反應（Polymerase chain reaction, PCR）方法調查台灣中部地區貓隻感染之Mycoplasma病原體，且進一步進行分子特性分析。本研究於100年3月至9月期間收集120隻於國立中興大學獸醫教學醫院及5間獸醫診所就診之貓隻抗凝血血液進行PCR檢驗及血液學分析。結果顯示共5（4.2%）隻貓隻為Mycoplasma haemofelis（Mhf）陽性，21（17.5%）貓隻為Candidatus Mycoplasma haemominutum（CMhm）陽性及2（1.6 %）隻貓隻為Candidatus Mycoplasma turicensis（CMt）陽性，3（2.5%）隻貓隻共同感染Mhf 及CMhm。感染Mhf的貓隻主要症狀包括貧血（80%）、厭食（100%）及精神抑鬱（80%）；感染CMhm的貓隻主要症狀包括貧血（90.5%）及精神抑鬱（81%）；而感染CMt的貓隻主要症狀包括貧血（100%）。此外，本研究推測公貓、混種貓及年齡低於6歲貓隻為感染CMhm的可能相關危險因子。進一步進行16S rRNA基因分析顯示5個Mhf序列與美國株序列相似度為100%，21個CMhm序列與英國及以色列株相似度為99.4-99.8%，2個CMt序列則分別與瑞士及南非株之相似度為100%。親緣關係樹狀圖顯示Mhf、CMhm及CMt皆聚集於相同種的族群。由本研究結果顯示台灣中部地區貓隻CMhm感染率最高，再來是Mhf及CMt
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