Effects of salinities on muscle amino acid and fatty acid composition of juvenile turbot(Scophthalmus maximus)

Abstract

为了探讨盐度对幼鱼肌肉中氨基酸和脂肪酸组成的影响,作者将平均体质量为(7.16±0.07)g的大菱鲆(SCOPHTHAl MuSMAXIMuS)幼鱼分别饲养在盐度(12、18、24、30(对照组)和36)水体中60 d。结果表明,不同盐度下,幼鱼肌肉中的氨基酸总量(TAA)、必需氨基酸(EAA)总量、非必需氨基(nEAA)总量和呈味氨基酸(fAA)总量分别在69.25%±0.37%~74.85%±1.26%、31.70%±0.11%~33.74%±0.28%、31.29%±0.44%~34.60%±1.26%和24.20%±0.66%~26.98%±1.02%,从高到低依次为36组>30组>12组>18组>24组,36组均显著高于24组(P0.05)。必需氨基酸指数(EAAI)在40.41±1.23~44.46±0.54,30和36组显著大于其他组(P30组>24组>12组>18组,各盐度组间均无显著性差异(P>0.05)。必需脂肪酸(EfA)总量在3.28%±0.04%~3.58%±0.10%,各盐度组间均无显著性差异(P>0.05)。饱和脂肪酸(SfA)总量在27.51%±0.37%~29.83%±0.35%,12组显著高于36组(P salinity 30 > salinity 12 > salinity 18 > salinity 24, and those of fish reared at salinity 36 were significantly higher than those of fish maintained at salinity 24(P0.05).The essential amino acid index(EAAI) was 40.41±1.23~44.46±0.54, increasing with the rise of water salinity.The EAAI of fish reared at salinities 30 and 36 was significant higher than those of other salinity groups(P salinity 30 > salinity 24 > salinity 12 > salinity 18, all of which had no significant differences between groups(P>0.05).The essential fatty acids(EFA) content was 3.28%±0.04%~3.58%±0.10%, no significant differences were observed between groups(P>0.05).The saturated fatty acids(SFA) content was 27.51%± 0.37%~29.83%±0.35%.The EFA in fish maintained at salinity 12 was significant higher than that of fish reared at salinity 36(P<0.05).Therefore, the qualities of free amino acid and fatty acid in muscles of fish reared at salinity 36 had been greatly improved in comparison with those of the control(salinity 30).This study provides important references for mechanistic researches on energy metabolisms of osmoregulation and salinity selection in turbot mariculture.国家现代农业产业技术体系建设专项资金资助项目(CA-RS-50