The microorganism Pseudomonas C12B was first isolated for its ability to degrade a wide range of anionic sulfate and sulfonate surfactants, and utilizes a number of n-alkanes and both primary and secondary aliphatic alcohols. In this work, ability to utilize n-alkanes was studied in more detail. After testing several cultivation arrangements, shaking in baffled Erlenmeyer flasks was chosen as the most appropriate for culturing Pseudomonas C12B on n-decane in liquid mineral medium. Mineralization of [1-14C] n-decane was demonstrated by production of radioactive carbon dioxide. The most effective inducers of n-decane utilization activity were (in descendent order) 2-decanol, 3-decanol, n-nonane, n-octane and 2-decanon. The microorganism harbors two enzyme systems that allow degradation of n-alkanes: a chromosomally encoded system specific to higher n-alkanes starting from n-dodecane and a plasmid encoded system is plasmid encoded and is specific towards medium-chain length n-alkanes. Size of the plasmid, designated pDEC, is between 200 and 300 kb. Its conjugative transfer was observed at high frequencies but only to the derivatives of Pseudomonas C12B previously cured of the plasmid. One of the genes responsible for degradation of n-alkanes was cloned in p. putida, where it was able to complement a mutation in the alkB gene situated on the OCT plasmid. Field experiments on bioremediation of soil polluted by petroleum wastes showed that the soil sample inoculated by Pseudomonas C12B was remediated faster in comparison with control experimentsAvailable from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi
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