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Estudos bioquimicos aplicados a taxonomia e a fisiologia das plantas parasitas nematoides

By Celia do Sacramento Santos Pais

Abstract

The present study included five populations of Meloidogyne naasi from different geographical areas, 47 populations of Meloidogyne species from Portugal and one population from Cabo Verde, nine populations of Globodera species and two populations of Heterodera avenae from Portugal. All the populations of M. naasi studied had characteristics consistent with M. naasi Franklin, 1965 and showed no obvious morphological or morphometric differences between the isolates. However, differences in the hatching behaviour and egg morality between the populations were found, so they were compared for their soluble protein and enzyme patterns of females in an attempt to detect intra specific variation between them. The technique used to separate the protein was micro-disc electrophoresis in gradient gels. To determine whether there was noticeable variation in the protein contents in the different developmental stages of the nematodes soluble proteins of egg masses and juveniles were also separated and compared with those of the females. Little variation was detected in protein and enzyme patterns among the populations. However, the pattern of soluble proteins from egg masses and second stage juveniles both differed from those found in adult females, each of the three development stages showing a characteristic protein pattern. After characterizing the populations of Meloidogyne spp. on the basis of differential host tests and perineal pattern morphology, soluble protein, nonspecific esterases, malate dehydrogenases, acid phosphatases, glucose - 6 - phosphate dehydrogenases, glutamate oxaloacetate transaminases and catalase isoenzymes of one to five females from the populations of root-knot nematodes were analyzed by electrophoresis in 0.4 mm thick polyacrylamide gels and isoelectric focusing. When using a sodium dodecyl sulphate (SDS) system to separate soluble proteins, clear patterns of bands were obtained from three adult females but, considering only the main bands of protein activity no apparent differences could be detected between populations of M. arenaria, M. incognita, M. javanica and M. hapla. Soluble protein profiles for different populations within each species..Available from Fundacao para a Ciencia e a Tecnologia, Servico de Informacao e Documentacao, Av. D. Carlos I, 126, 1200 Lisboa / FCT - Fundação para o Ciência e a TecnologiaSIGLEPTPortuga

Topics: 06W - Botany, 06A - Biochemistry
Year: 1990
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Provided by: OpenGrey Repository
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