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INFLUENCE OF HYDROPHOBIC AMINO ACIDS (TRP) IN MATURE PART OF BINASE ON ITS SECRETION

By Leonid Valentinovich Lopukhov and Kazan Scientific Centre of the Russian Academy of Sciences (Russian Federation) The Kazansky Institute of Biology

Abstract

The investigation object is the extracellular alkaline ribonuclease Basullus intermedius (binase). The aim is to reveal the mechanism of influencing hydrophobic amino acids in the mature part of the secreted proteins on their secretion as an example of the binase, in comparison with prepromelitin and growth hormone of the man. It has been determined that the substitution of the hydrophobic tryptophan in the mature part of the binase leads to the blocking of its secretion in the recombinant strains of E. coli. The performed analysis of the binase structure and comparison with the results obtained for other proteins permits to conclude that the most probable cause of blocking secretion of the binase having the substitutions on the tryptophan is that the presecretory protein cannot receive the conformation abble to the translocation. The comparative computer analysis of the polypeptide structures influencing on their secretion, for such proteins as the prepromelitin, growth hormone and binase has been made. It has been discovered that the parts of the mature sequences in these proteins influencting on their secretion have the amphiphilic secondary structure. It has been shown that the substitution of the tryptophan residues in the mature part of the binase blocks its secreation in the cells of E. coli.Available from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio

Topics: 07C - Organic chemistry, HYDROPHOBIC AMINO ACIDS, BINASE, SECRETION
Year: 1997
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Provided by: OpenGrey Repository
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