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FORMATION OF 8-OXYGUANYLIC NUCLEOTIDES UNDER ACTION OF GAMMA-RADIATION AND INFLUENCE OF 8-OXY-GUANOSINE DIPHOSPHATE (GDP) ON SYNTHESIS OLIGONUCLEOTIDES IN DNA-POLYMERASE REACTION

By Anna Maximovna Usachyeva

Abstract

The formation of 8-oxyguanylic derivatives at gamma-radiation of the nucleoside and nucleotide solutions (Guo, dGuo, GMP, dGMP, GDP and GTP) has been investigated. At irradiation of the nucleosides in H*002O and in D*002O it has been discovered that under of the gamma-irradiation and heat the mechanisms of forming B-oxyguanine are differed each from other. At 37 degrees C the damage level in the cellular fund of GTP induced by a natural radiation background constitutes 0,2% from heat one but at 4 degrees C - 20%. The methodical approach to the evaluation of the radiation and heat damage in the nucleotides by a differential UV-spectroscopy has been approved. The substrate properties of 8-oxy-GTP and 8-Br-GTP in the synthesis reaction of the oligonucleotides by DNA-polymerase E.coli on the promotor A1 DNA of the phage T7 DELTA D111 at limited set of the substrates have benn investigated, and the inhibition kinetics has been considered. The qualitative characteristics of bonding these analogs by RNA-polymerase have been calculated. 8-oxy-GTP and 8-Br-GTP compete with ATP but in comparison with ATP are not included into the product constitution. 8-oxy-GTP competes with UTP and is capable to substitute UTP at synthesis of the tetra- and pentanucleotide. A possibility to form the abnormal pair of 8-oxyG-A at synthesis of the ribonucleis acids has been shown firstly. 8-oxy-GTP and 8-Br-GTP are uncapable to be the initiator nucleotides at synthesis on the weak D-promotor. In the system of the synthesis with polynucleotide phosphorylase the polynucleotide polyAoxoG (30 - 100 chains) and polyoxoG (30 - 70 nucleotides) has been synthesizedAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio

Topics: 07E - Nuclear chemistry, radiochemistry, CHEMISTRY
Year: 1995
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Provided by: OpenGrey Repository
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