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Molekularbiologische Untersuchungen zur Glutamataufnahme in Zymomonas mobilis und Escherichia coli

By N. Peekhaus and Forschungszentrum Juelich G.m.b.H. (KFA) (Germany). Inst. fuer Biotechnologie

Abstract

Investigations on the structure, mechanism and evolution of the bacterial periplasmic transport system have been carried out on Z. mobilis and E. coli. During this work a glutamate/aspartate uptake system and the regulator grp (glutamate uptake regulary protein) have been isolated from Z. mobilis and subjected to a detailed characterization. Several regulary functions of the grp could be proved in E. coli. Gpr acts in a leucine-independent manner as an activator of the ilvIH operon, and it enhances the expression of a glutamate uptake system. At the same time it was demonstrated that grp reprimizes the expression of the glutamate/aspartate uptake system GluEMP isolated from Z. Moblis. Expression of grp in Z. mobilis and E. coli was studied by means of lacZ-mobilis and E. colie was studied by means of lacZ-gene fusion. In these experiments also a new regulatory function of the lrp was found: lrp enhances the expression of the binding protein-dependent glutamate uptake system of E. coli. (WEN)SIGLEAvailable from TIB Hannover: RA 831(3000) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Topics: 06B - Bioengineering, biomedical engineering, biotechnology, biochemical engineering, GENETIC FUNDAMENTALS: M, ZYMOMONAS MOBILIS, ESCHERICHIA COLI, GLUTAMAT INTAKE, GLUTAMIN INTAKE, FACTOR REGULATION, TRANSPORT PROCESSES
Year: 1994
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Provided by: OpenGrey Repository
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