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Entwicklung eines Immunosensors auf der Basis akustischer Oberflaechenwellen

By T. Wessa, Forschungszentrum Karlsruhe GmbH Technik und Umwelt (Germany). Projekt Mikrosystemtechnik and Forschungszentrum Karlsruhe GmbH Technik und Umwelt (Germany). Inst. fuer Instrumentelle Analytik

Abstract

In this paper the use of commercially available surface acoustic wave devices for in-situ measurements in water was investigated. This was achieved by the use of piezoelectric substrates on the base of 36 -rot Y, X, LiTaO_3 and horizontally polarized surface transverse waves. The devices worked as frequency determining elements of an oscillator circuit. The mass change caused by the precipitation of biomolecules was detected by the change of the resonance frequency of the oscillator. The interdigital transducers of the devices were made of aluminium because of its excellent acoustic characteristics. However, the chemical instability of aluminium led to enormous corrosion processes even in phosphate buffer solution (pH=7). A thin shielding layer of polyimide showed the best properties to prevent these reactions while facilitating covalent immobilization of antibodies for the preparation of immunosensors. The dependence of the sensor signal caused by a change of conductivity and viscosity was investigated with shielded devices. This shielding technique seems to be very appropriate to serve as a base for further steps towards an immunosensor. Two different procedures of covalent attachment of antibodies were developed. (orig.)SIGLEAvailable from TIB Hannover: ZA 5141(5803) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Topics: 07D - Physical chemistry, IMMUNOSENSORS: M, IMMUNO CHEMICAL ACTIVITY, SURFACE WAVES, PHOTOREACTION, SURFACE ACOUSTIC WAVE DEVICES, PROTECTION LAYERS, CONSTRUCTION PARTS, PHOTOIMMOBILIZATION METHOD
Year: 1996
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Provided by: OpenGrey Repository
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