Entwicklung und Erprobung einer kombinierten, computergesteuerten Puls-Feld-und Feld-Inversions-Gelelektrophorese-Apparatur Schlussbericht

Abstract

For automatic quantitative evaluation and control of pulse field gel electrophoresis (PFGE) a configuration of video camera optical system, computer-based image analysis, PFGE/FJGE-apparatus and power supply is described. Software is presented permitting detection, automatic reading and data processing of low-light and low contrast DNA-gels and of autoradiographs. The following options are available: Automatic molecular weight and concentration calculation; introducing a databank of chromosomal DNA molecules; reproducible, standardized comparisons of different complex electrophoretic patterns (lanes, gels, autoradiographs); identification of unknown DNA-fragments and locating of similarities; chromosome mapping; control of genetic stability in microorganism; documentation-managingSIGLEAvailable from TIB Hannover: F93B40 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekArbeitsgemeinschaft Industrieller Forschungsvereinigungen e.V., Koeln (Germany)DEGerman