The Seine River is strongly affected by the effluents from the Achères wastewater treatment plant (WWTP)\ud downstream of the city of Paris. We have shown that the effluents introduce large amounts of ammonia and\ud inoculate the receiving medium with nitrifying bacteria. The aim of the present study was to investigate the\ud diversity of the ammonia-oxidizing bacterial population by identifying autochthonous bacteria from upstream\ud and/or allochthonous ammonia-oxidizing bacteria from the WWTP effluents. Measurements of potential\ud nitrifying activity, competitive PCR, and denaturing gradient gel electrophoresis (DGGE) of 16S ribosomal\ud DNA fragments specific to ammonia-oxidizing bacteria (AOB) were used to explore the succession and shifts\ud of the ammonia-oxidizing community in the lower Seine River and to analyze the temporal and spatial\ud functioning of the system at several different sampling dates. A major revelation was the stability of the\ud patterns. The CTO primers used in this study (G. A. Kowalchuk, J. R. Stephen, W. D. Boer, J. I. Prosser, T. M.\ud Embley, and J. W. Woldendorp, Appl. Environ. Microbiol. 63:1489–1497, 1997) were shown not to be completely\ud specific to AOB of the B subclass of Proteobacteria. We further demonstrated that when DGGE patterns are\ud interpreted, all the different bands must be sequenced, as one major DGGE band proved to be affiliated with\ud a group of non-AOB in the B subclass of Proteobacteria. The majority of AOB (75 to 90%) present in the lower\ud Seine river downstream of the effluent output belong to lineage 6a, represented by Nitrosomonas oligotrophaand\ud Nitrosomonas ureae-like bacteria. This dominant lineage was represented by three bands on the DGGE gel.\ud The major lineage-6a AOB species, introduced by the WWTP effluents, survived and might have grown in the\ud receiving medium far downstream, in the estuary; it represented about 40% of the whole AOB population. The\ud other two species belonging to lineage 6a seem to be autochthonous bacteria. One of them developed a few\ud kilometers downstream of the WWTP effluent input in an ammonia-enriched environment, and the other\ud appeared in the freshwater part of the estuary and was apparently more adapted to estuarine conditions, i.e.,\ud an increase in the amount of suspended matter, a low ammonia concentration, and high turnover of organic\ud matter. The rest of the AOB population was represented in equal proportions by Nitrosospira- and Nitrosococcus\ud mobilis-like species
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