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Continuous culture enrichments of ammonia-oxidizing bacteria at low ammonium concentrations

By A. Bollmann and H.J. Laanbroek

Abstract

Until now enrichments of ammonia-oxidizing bacteria from natural ammonium-limited environments have been performed mainly in the\ud presence of much higher ammonia concentrations than those present in the natural environment and many have resulted in the enrichment\ud and isolation of environmentally less important bacteria. Therefore, we used continuous cultures to enrich ammonia-oxidizing bacteria at\ud growth-limiting ammonium concentrations of around 5 UM from the root zone of the macrophyte Glyceria maxima from the lake\ud Drontermeer (The Netherlands). Molecular analysis at the end of the enrichment experiments showed that all continuous cultures consisted\ud of Nitrosomonas cluster 6a, which comprises also Nitrosomonas ureae and Nitrosomonas oligotropha. This was independent of whether\ud Nitrosomonas- or Nitrosospira-like bacteria were dominant in the inoculum. Thus all known ammonia-oxidizing bacteria belonging to\ud Nitrosomonas cluster 6a are able to grow at very low ammonium concentrations

Topics: Biologie, Nitrification, Ammonia oxidation, Substrate limitation, Chemostat, Enrichment, DGGE
Year: 2001
OAI identifier: oai:dspace.library.uu.nl:1874/25551
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