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Purification of free eukaryotic initiation factors eIF-4A and eIF-4D on cibacron blue F3G-A

By C. van der Mast and H.O. Voorma

Abstract

The eukaryotic initiation factors eIF-4A and eIF-4D are almost exclusively found in the supernatant of a reticulocyte lysate. The basic steps of general purification schemes failed because these factors do not bind to Sepharoseheparin or, in the case of eIF-4A, to phosphocellulose and a new procedure had to be devised. Cibacron Blue F3G-A proved to be a successful alternative in the purification of these factors from the excessive load of proteins found in the supernatant

Topics: Biologie, initiation factor purification, Cibacron Blue, rabbit
Year: 1980
OAI identifier: oai:dspace.library.uu.nl:1874/24469
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