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The localisation of phoE and β-galactosidase antigens in wild type and phoE/lacZ hybrid Escherichia coli

By A. Verkleij, J. Leunissen, M. van Damme-Jongsten, J.P.M. Tommassen and B. Lugtenberg


PhoE, one of the outer membrane proteins\ud in E. coli K-12 cells, is involved in the\ud uptake of small hydrophilic, especially\ud negatively charged solutes. Its synthesis\ud is induced upon phosphate limitation. Several\ud models have been proposed for the mechanism\ud of transport and incorporation of\ud outer membrane proteins into the outer membrane. Escherichia coli cells containing\ud a hybrid phoE/lacZ gene were studied\ud to investigate the destination of the hybrid\ud gene product, using an immunocytochemical\ud labeling procedure and ultra-thin\ud cryosectioning

Topics: Biologie
Year: 1984
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