Pheochromocytoma cells (clone PC12)
have specific plasmamembrane receptors
for both epidermal growth factor (EGF)
and nerve growth factor (NGF).
These growth factors have however, opposite
biological effects in PC12 cells;
EGF acts mitogenically, while NGF induces
differentiation and causes arrest
of cell proliferation.
EGF-receptor interaction in PC12
cells was characterized biochemically
by ¹²⁵I-EGF binding studies. EGF binding
was concentration-dependent and
saturable. Scatchard graph analysis
indicated that two different classes
of EGF binding sites may be present in
PC12 cells. Addition of NGF to PC12
cells caused a reduction in EGF binding,
the result of a decrease of the number
of binding sites and in their affinity
for EGF. The reduction of EGF binding
was dependent upon the duration and
concentration of NGF exposure
Is data on this page outdated, violates copyrights or anything else? Report the problem now and we will take corresponding actions after reviewing your request.