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Lateral mobility of plasma membrane lipids in Xenopus eggs: Regional differences related to animal/vegetal polarity

By S.W. de Laat, J.G. Bluemink, W.J.A.G. Dictus, E.J.J. van Zoelen, P.A.T. Tetteroo and L.G.J. Tertoolen


Regional differences in the lateral\ud mobility properties of plasma membrane\ud lipids were studied in unfertilized and\ud fertilized Xenopus eggs by fluorescence\ud photobleaching recovery (FPR) measurements.\ud Out of a variety of commonly used\ud lipid probes only the aminofluorescein-\ud -1abelled fatty acids HEDAF (5-(N-hexadecanoyl)-\ud aminofluorescein) and TEDAF\ud (5-(N-tetradecanoyl)-aminofluorescein)\ud appear to distribute itself in the plasma\ud membrane. Under all experimental conditions\ud used these molecules show partial\ud recovery upon photobleaching, indicating\ud the existence of lipidic microdomains

Topics: Biologie
Year: 1984
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