Skip to main content
Article thumbnail
Location of Repository

Lateral mobility of plasma membrane lipids in dividing Xenopus eggs

By S.W. de Laat, P.A.T. Tetteroo, J.G. Bluemink, W.J.A.G. Dictus and E.J.J. van Zoelen


The lateral mobility of plasma membrane lipids was analyzed during first cleavage of Xaopus Levis eggs by\ud fluorescence photobleaching recovery (FPR) measurements, using the lipid analogs 5-(N-hexadecanoyl)aminofluorescein\ud (“HEDAF”) and 5-(N-tetradecanoyl)aminofluorescein (“TEDAF”) as probes. The preexisting plasma membrane of the\ud animal side showed an inhomogeneous, dotted fluorescence pattern after labeling and the lateral mobility of both\ud probes used was below the detection limits of the FPR method (D < 10⁻¹⁰ cm²/sec). In contrast, the preexisting plasma\ud membrane of the vegetal side exhibited homogeneous fluorescence and the lateral diffusion coefficient of both probes\ud used was relatively high (HEDAF, D = 2.8 X 10⁻⁸ cm²/sec; TEDAF, D = 2.4 X 10⁻⁸ cm²/sec). In the cleaving egg visible\ud transfer of HEDAF or TEDAF from prelabeled plasma membrane to the new membrane in the furrow did not occur,\ud even on the vegetal side. Upon labeling during cleavage, however, the new membrane was uniformly labeled and both\ud probes were mobile, as in the vegetal preexisting plasma membrane. These data show that the membrane of the\ud dividing Xenopus egg comprises three macrodomains: (i) the animal preexisting plasma membrane; (ii) the vegetal\ud preexisting plasma membrane; (iii) the new furrow membrane

Topics: Biologie
Year: 1984
OAI identifier:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • (external link)
  • Suggested articles

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.