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Determination of specific anti-leptospiral immunoglobulins M and G in sera of experimentally infected dogs by solid-phase enzyme-linked immunosorbent assay

By J.F. Frik, E.G. Hartman, M. van Houten and J.A. van der Donk


The development and evaluation of an enzyme-linked immunosorbent assay\ud (ELISA) to detect specific anti-leptospiral IgM and IgG in sera of dogs experimentally\ud infected with Leptospira interrogans serotype canicola are reported.\ud In all dogs specific anti-leptospiral IgM was detected from the second half of\ud the first week after infection, the maximum being attained during the second\ud week. Subsequently the IgM titre gradually decreased. Specific anti-leptospiral\ud IgG was detected later and increased gradually to reach almost the same level\ud as the IgM titre after two to three months.\ud During the initial stage of the infection, when the microscopic agglutination\ud titre was still negative or very low, a high IgM titre was accompanied by a\ud negative or very low IgG titre in every case. After the initial stage a substantial\ud IgG titre was also detectable. It is suggested that the test is suitable\ud for serodiagnostic purposes, particularly for the diagnosis of a current infection\ud in an individual

Topics: Diergeneeskunde
Year: 1984
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