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Probing In Vivo Promoter Activities in Mycoplasma pneumoniae: a System for Generation of Single-Copy Reporter Constructs

By Sven Halbedel and Jörg Stülke


The nucleotide sequences that control transcription initiation and regulation in Mycoplasma pneumoniae are poorly understood. In this work, we developed a lacZ-based reporter plasmid that can be used to integrate fusions of promoter fragments to a promoterless lacZ gene into the chromosome of M. pneumoniae

Topics: Genetics and Molecular Biology
Publisher: American Society for Microbiology
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Provided by: PubMed Central
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