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Properties of Allyl Alcohol-Resistant Mutants of Clostridium butyricum Grown on Glycerol

By S. Abbad-Andaloussi, J. Amine, P. Gerard, E. Petitdemange and H. Petitdemange

Abstract

Mutants of Clostridium butyricum E5 exhibiting resistance to allyl alcohol which produced the same quantities of 1,3-propanediol as the wild-type strain but more acetate than butyrate were isolated. The acetate-butyrate formation plays a major function in the regulation of the internal redox balance. Allyl alcohol resistance can be attributed not to the loss of 1,3-propanediol dehydrogenase but to a shift in the reductive properties of the enzyme. The data support the view that cellular regulation is modified to avoid intracellular accumulation of 3-hydroxypropionaldehyde

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OAI identifier: oai:pubmedcentral.nih.gov:1388949
Provided by: PubMed Central
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