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Proteomics of cryoprotective dehydration in Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola)

By M.A.S. Thorne, M.R. Worland, R. Feret, M.J. Deery, K.S. Lilley and Melody Clark

Abstract

The Arctic springtail, Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola), is one of the few organisms known to survive the extreme stresses of its environment by using cryoprotective dehydration. We have undertaken a proteomics study comparing M. arctica, acclimated at -2 degrees C, the temperature known to induce the production of the anhydroprotectant trehalose in this species, and -6 degrees C, the temperature at which trehalose expression plateaus, against control animals acclimated at +5 degrees C. Using difference gel electrophoresis, and liquid chromatography tandem mass spectrometry, we identified three categories of differentially expressed proteins with specific functions, up-regulated in both the -2 degrees C and -6 degrees C animals, that were involved in metabolism, membrane transport and protein folding. Proteins involved in cytoskeleton organisation were only up-regulated in the -6 degrees C animals

Publisher: Wiley
Year: 2011
DOI identifier: 10.1111/j.1365-2583.2010.01062.x
OAI identifier: oai:nora.nerc.ac.uk:16633
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