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Safe Mycobacterium tuberculosis DNA Extraction Method That Does Not Compromise Integrity

By Robin Warren, Marianna de Kock, Erica Engelke, Roseline Myburgh, Nico Gey van Pittius, Thomas Victor and Paul van Helden

Abstract

Concern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist

Topics: Mycobacteriology and Aerobic Actinomycetes
Publisher: American Society for Microbiology
OAI identifier: oai:pubmedcentral.nih.gov:1351970
Provided by: PubMed Central
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