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Glycoconjugates of the lateral prostate of the guinea pig: A lectin histochemical study

By YC Wong and L Chan


The present study examined the glycoconjugates of the lateral prostate using a battery of lectins. The results indicated that the secretory epithelium was rich in mannose (Man), N-acetylglucosamine (GlcNAc), galactose (Gal), N-acetylgalactosamine (GalNAc), and complex oligosaccharides. Con A (concanavalin A), LCA (Lens culinaris agglutinin), PSA (Pisum sativum agglutinin), WGA (wheat germ agglutinin), PWM (pokeweed mitogen), RCA-I (Racinus communis isolectin I), and PHA-P (Phaseolus vulgaris agglutinin-P) reacted intensely with both epithelia and stroma, while SBA (soybean agglutinin) and PNA (peanut agglutinin), which bind to terminal Gal, GalNAc, and Gal β1,3 GalNAc appeared to be specific to the secretory epithelium. SBA and PNA were useful as markers in the study of the secretory function of the prostate gland. The present study has shown that the Golgi apparatus of prostatic epithelial cells was rich in fucose (Fuc), oligomers of GlcNAc, Gal β1,3 GalNAc, Gal, and Man containing glycoconjugates, indicating that the gland was actively involved in glycosylation. The present study has also shown that LTA (Lotus tetragonolobus agglutinin) is a good marker for the epithelial Golgi. PNA also bound to the epithelial basement membrane and the connective tissue in the lamina propria. The present study has thus established, for the first time, the glycoconjugate patterns in the lateral prostate of the guinea pig.link_to_subscribed_fulltex

Topics: Acetylgalactosamine - Biosynthesis, Acetylglucosamine - Biosynthesis, Agglutinins - Diagnostic Use, Animals, Concanavalin A - Diagnostic Use, Epithelium - Metabolism, Extracellular Matrix - Chemistry, Galactose - Biosynthesis, Glycoconjugates - Analysis, Guinea Pigs, Immunohistochemistry, Lectins - Diagnostic Use, Male, Mannose - Biosynthesis, Plant Lectins, Pokeweed Mitogens - Diagnostic Use, Prostate - Metabolism
Publisher: 'United States Sports Academy'
Year: 1991
DOI identifier: 10.1002/pros.2990190209
OAI identifier:
Provided by: HKU Scholars Hub
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